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Avidin
It is a strongly basic glycoprotein synthesized in the hen oviduct and deposited in the albumen fraction of eggs at a ratio of 0.05% of the total protein content. It binds the water-soluble vitamin H (d-biotin) very tightly and specifically with a binding log constant of 15. The constant high affinity of Avidin for Biotin has been widely used in molecular biology: affinity chromatography, molecular recognition and labelling, Enzyme Linked ImmunoSorbent Assay (ELISA), histochemistry and cytochemistry. It is largely used in medical diagnostic kits. The purification process used by Belovo to produce Avidin (affinity chromatography) guarantee an Avidin totally active with the highest purity. |
